After incubated for 10 d, the cells were fixed with methanol for 15 min at 25C and visualized by staining with 1% methylene blue. Tumor Spheroid-Formation Assays Inhibition of tumor spheroid-formation by Compact disc44+ cells following treatment with various Gli1 siRNA formulations was determined using the tumor spheroid tradition technique. multipoint conjugates, DSPE-HA single-point conjugates … Continue reading After incubated for 10 d, the cells were fixed with methanol for 15 min at 25C and visualized by staining with 1% methylene blue